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Confocal calcium imaging reveals an ionotropic P2 nucleotide receptor in the paranodal membrane of rat Schwann cells

机译:共聚焦钙成像显示大鼠雪旺氏细胞旁淋巴结膜中的离子型P2核苷酸受体

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摘要

The paranodal Schwann cell region is of major importance for the function of a myelinated axon. In the present study we searched for a possible ionotropic effect of extracellular ATP in this Schwann cell compartment.Whole-cell patch-clamp recordings from cultured rat Schwann cells revealed that ATP and 2′–3′-O-(4-benzoylbenzoyl)-adenosine 5′-triphosphate (BzATP) induced a non-specific cation current. The effect of ATP was much enhanced in a Ca2+- and Mg2+-free solution. ADP, UTP and α,β-methylene adenosine 5′-triphosphate (α,β-meATP) had no effect.Confocal Ca2+ imaging of myelinating Schwann cells in isolated rat spinal roots showed a BzATP-induced rise in the free intracellular Ca2+ concentration in the paranodal Schwann cell cytoplasm whereas α,β-meATP and 2-(methylthio)-adenosine 5′-triphosphate were without effect. In contrast to the known metabotropic effect of UTP on these Schwann cell regions, the BzATP-induced Ca2+ signal was not transient, was unaffected by depletion of intracellular Ca2+ stores and dependent on the presence of extracellular Ca2+.These results suggest that an ionotropic ATP receptor with electrophysiological and pharmacological characteristics of the P2X7 subtype of nucleotide receptors is functionally active in myelinating Schwann cells of peripheral nerves. Such a receptor might contribute to Schwann cell reactions in nerve injury or neuropathy.
机译:偏旁的雪旺氏细胞区域对于髓鞘轴突的功能至关重要。在本研究中,我们搜索了该雪旺氏细胞隔室中细胞外ATP的可能的离子作用。培养的大鼠雪旺氏细胞的全细胞膜片钳记录显示,ATP和2'–3'-O-(4-苯甲酰基苯甲酰基)-腺苷5'-三磷酸(BzATP)诱导了非特异性阳离子电流。在不含Ca2 +和Mg2 +的溶液中,ATP的作用大大增强。 ADP,UTP和α,β-亚甲基腺苷5'-三磷酸(α,β-meATP)无作用。离体大鼠脊髓根部有髓神经鞘雪旺细胞的Ca2 +显像显示BzATP诱导的大鼠游离细胞内Ca2 +浓度升高淋巴结的施旺细胞细胞质,而α,β-meATP和2-(甲硫基)-腺苷5'-三磷酸没有作用。与UTP对这些Schwann细胞区域的已知的促代谢作用相反,BzATP诱导的Ca2 +信号不是瞬时的,不受细胞内Ca2 +储存耗竭的影响并且取决于细胞外Ca2 +的存在。这些结果表明,离子型ATP受体具有P2X7亚型核苷酸受体的电生理和药理学特征的人在周围神经的髓鞘雪旺细胞中具有功能活性。这种受体可能在神经损伤或神经病中促进雪旺细胞反应。

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